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Prime 2019 Prime2019Full Release6 November 2018Custom Codon Usage TablesBack translate and optimize codons using your own codon usage tables. R11 R11FullRelease3 October 2017Volcano Plots for RNA-Seq Expression AnalysisVisualize gene expression in an interactive volcano plot that can be used to highlight and jump to differentially expressed genes.Pretty PCA PlotsCreate beautiful PCA plots with better labelling and new visualization options.Silent Mutation Analysis for Restriction SitesAutomatically identify point mutations in a coding sequence that will introduce a restriction site without affecting the protein.CRISPR Cpf1 OptionAdded support for CRISPR-Cpf1 with a 5’ PAM site. Find sites in A and T rich sequences and with higher cleaving efficiency in vivo.Create Enzyme SetsWhat’s in the freezer?
No longer pull information from other sources. Now you can easily create your own enzyme sets.Better FASTQ ImportPaired reads can be associated with each other during import and Geneious will do it’s best to guess how they’re paired. R10 R10FullRelease4 October 2016Smarter Restriction CloningCompletely redesigned to be much simpler and more powerful.
Includes automatic identification of compatible cut sites, and digestion and ligation with drag and drop ordering of fragments.Alignment MaskingMask unreliable alignment sites for better tree building, without deleting data. Manage alignment masking patterns using annotation tracks and automatically mask sites according to user defined criteria.Improved Plasmid ViewerThe new circular overview option helps you stay oriented by showing the bases of the sequence and the plasmid map alongside each other with synchronized editing, selection and scrolling.Chimera FilteringFilter chimeric reads by comparing to a reference database, which can be any sequence list or alignment. Choose between the bundled public domain UCHIME algorithm or the faster USEARCH implementation.Lineage View for Parent/DescendantsLineage View is now easier to access as a separate tab and details about your cloning operations are shown right next to the relationships.Assembly and MappingBetter structural variant mapping, a new workflow to build SNP trees by applying variants to a reference and better de novo assembly of Ion Torrent, 454, and PacBio CCS data.R10.1 Update7 February 2017Improved CloningGibson and Golden Gate now has sequence view of fragments within the options. Gibson assembly can now saves primers for easy orderingAnnotate from DatabaseAnnotate nucleotide sequences from a protein database by comparing translations of the nucleotide sequence in all six frames with the protein database sequencesZip ImportZip files containing multiple files and sub-folders can now be importedSequence ViewerAdded ‘Copy Translation’ to translate selected regions of a nucleotide sequence (in right-click menu)SPAdes de novo assemblerFor MacOS/Linux: 64 bit OS is required. For Windows, 64 bit Windows 10 (with recent updates installed) is required.
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You also need to install additional Windows features (Instructions)Find CRISPR SitesMajor performance improvements were made to the off-target scoring algorithmAll CRISPR sites will now be scored against all potential off-target sites in every runScoring CRISPR sites against off-targets now takes a seed region into account. This is a 10 bp region adjacent to the PAM site that can tolerate a maximum of 2 mismatches and 0 indelsR10.2 Update6 June 2017RNA-Seq Expression AnalysisCompare Expression Levels now has the option to use DESeq2 for pairwise analysis with replicate samples and will produce a principle component analysis (PCA) plot for quality control.Cloning WorkflowsRestriction Cloning, Gibson Assembly and Golden Gate can now be incorporated into Workflows. Automate your cloning procedures or perform high-throughput batch cloning. R9 R9FullRelease7 October 2015Golden GateIntelligent handling of preconfigured Golden Gate parts and automatic designing of primers for new parts.RNA- Seq and Structural Variant MappingMap RNA-seq using existing annotated coding regions or discover novel intron and fusion genes during mapping. For DNA sequencing, you can now discover structural variants and annotate them, allowing for correct alignments of read-ends around deletions and structural rearrangements in heterozygous samples.Big TreesNew search box for finding nodes and taxa, and automatic collapsing of sub-trees based on distances. Greatly improved performance when loading and rendering trees.Cutting edge CRISPRNew scoring strategy for on-target activity. R7 R7FullRelease3 September 2013WorkflowsEasily create and share workflows based on your data and analysis.
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Try some of the built-in workflows or design your own.Gibson AssemblyOne-step cloning of fragments using Gibson assembly including automated primer design and batch cloning/shufflingCodon OptimizationFind rare codons or produce a fully optimized sequence based on a target organism and remove unwanted restriction sites at the same time.TOPO CloningOne-step cloning of fragments into a vector using TOPOCopy and Paste AnnotationsAnnotations are now maintained when copying sequences! R6 R6FullRelease4 October 2012Latest Primer3 engineIncluding brand new thermodynamic calculations that give you better stats on primers.Automatic plasmid annotationNo more manual searching. New automatic plasmid annotation lets you sort through your data faster than ever before, saving your time and your eyesight.Read MappingNew fine-tuning algorithm quickly generates accurate results using less memory.Enhanced Variant and SNP callingEliminate false variants, annotate average quality, find variations within specified annotations. Geneious R10.2.Geneious R10.1.Geneious R10.Geneious R9.1.Geneious R9.Geneious R8.1.Geneious R8.Geneious R7.1.Geneious R7.Geneious R6.1.Geneious R6.Geneious 5.6.Geneious 5.5.Geneious 5.4.Geneious 5.3.Geneious 5.1.Geneious 5.0.Geneious 4.8.Geneious 4.7.Geneious 4.6.Geneious 4.5.Geneious 4.0.Geneious 3.8.Geneious 3.7.Geneious 3.6.Geneious 3.5.Geneious 3.0.Geneious 2.5.Geneious 2.0.